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Antibodies

ATM phospho S1981 Antibody

Product Sizes
100 ug
200-301-500-100UG
About this Product
SKU:
200-301-500
Additional Names:
ATM|mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody
Application:
Detection/Quantitation, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
7C10D8
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Anti ATM pS1981 Antibody recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370-kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint; Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest; and Brca1 and hRad17 in the G2/M checkpoint. See Bakkenist, C. J. & Kastan, M. B. Nature 421, 499-506 (2003) for a complete presentation of this antibody's specificity and utility.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
This antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.
Isotype:
IgG2a
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This Protein A Purified Mab antibody is directed against human ATM and is useful in determining its presence by immunohistochemistry. This monoclonal anti-ATM antibody recognizes the phosphorylated form of the protein in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human and mouse ATM. Cross reactivity with ATM from other mammalian source
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Anti ATM monoclonal Antibody was used to show constitutive activation of the ATM pathway in human urinary bladder cancer. Anti-ATM Protein Kinase pS1981 (clone # 7C10D8 ) was used to stain normal uroepithelium, early superficial lesions (Ta), and earliest invasive (T1) primary carcinomas. ATM protein is ubiquitously expressed, but Ser 1981-phosphorylated ATM (pS-ATM) is detectable only in tumor tissues. See Bartkova et al. for additional details. Reprinted by permission from Nature (Cell Cycle 4;(6), 2005) Macmillan Publishers Ltd.
ATM authophosphorylation.
Determination of ATM and ATMp antibodies specificity by western blotting. Western blot analysis of neuroretinas and brains from adult Atm-deficient and adult C57BL/6J mice was performed with monoclonal antibodies raised against ATM and ATMp, and with polyclonal antibody B Beta-actin (internal control). Specific bands for ATM (350 kDa), ATMp (350 kDa), and B Beta-actin (35 kDa) were detected by western blot analysis of protein homogenates extracted from different mouse tissues (neuroretina and brain). No band could be detected in protein homogenates from Atm-deficient mice tissues blotted with either specific ATM or specific ATMp antibody. Figure provided by CiteAb. Source: Mol Vis, PMID: 19234633.