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Antibodies

Anti-PDK1 (N-terminus) Antibody

Product Sizes
100 ul
PP1411-100UL
About this Product
SKU:
PP1411
Additional Names:
PDPK1, PKBK
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our PDK1 (N-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat PDK1 (N-terminus) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
PDK1
Host:
Rabbit
Immunogen:
PDK1 synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminus of human PDK1. This sequence is highly conserved in rat and mouse PDK1.
Isotype:
IgG
Molecular Weight:
65
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 65 kDa* protein corresponding to the apparent molecular mass of PDK1 on SDS-PAGE immunoblots of human A431 cells and mouse brain.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
Western blot image of A431 cells untreated (lanes 1 and 3) or treated with pervanadate (lanes 2, 4, 5 & 6). Blots were probed with anti-PDK1 (PP1411) or anti-PDK1 (Tyr-9) (PP1431). The latter was used in the presence of no peptide (lane 4), phospho-PDK1 (Tyr-9) peptide (lane 5), or an unrelated phosphotyrosine peptide (lane 6).