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Assay Kits & ELISAs

Fluoro Catalase Activity Detection Kit by Cell Technology

Product Sizes
500 tests
FLOCAT100-3-500TESTS
About this Product
SKU:
FLOCAT100-3
Additional Names:
Metabolic Assay
Application:
ELISA
Extra Details:
A sensitive, one-step, no wash assay for the sensitive monitoring of catalase activity by measuring residual hydrogen peroxide substrate. A non-fluorescent detection reagent generates a fluorescent signal in the presence of peroxidase and resorufin.
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Detection
Figure 1. Assay principle
Figure 2. The reaction contained 20mM hydrogen peroxide (final) per well and the indicated amounts of catalase. The reaction was incubated for 30 minutes at room temperature. Reaction cocktail was added to each well and the reaction incubated for another 10 minutes in the dark at room temperature. Fluorescence was measured at excitation 530nm and emission 590nm. The graphs show the net fluorescence change: Negative Control fluorescence (no catalase) minus Sample (catalase) fluorescence.