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Antibodies

ATG2A Antibody: PerCP

Product Sizes
100 ug
SPC-652D-PCP-100UG
About this Product
SKU:
SPC-652D-PCP
Additional Names:
ATG2 autophagy related homolog A Antibody, ATG2a_HUMAN Antibody, BC023754 Antibody, KIAA0404 Antibody, 1810013C15Rik Antibody
Application:
Immunocytochemistry, Immunofluorescence, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
PerCP
Concentration:
1 mg/ml
Extra Details:
ATG2A belongs to the ATG2 family. It is required for both autophagosome formation and regulation of lipid droplet morphology and dispersion (1).
Host:
Rabbit
Immunogen:
Synthetic peptide from the C-terminal of human ATG2A
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Specificity:
Predicted molecular weights at ~213kDa. Other observed bands around ~90-100kDa to be expected.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Polyclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG2A Antibody (D) Composite.
Western blot analysis of Mouse Brain showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (SPC-652) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.