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Antibodies

ASAH1 Antibody

Product Sizes
100 ug
600-401-Y65-100UG
About this Product
SKU:
600-401-Y65
Additional Names:
ASAH1|ASAH1 Antibody, AC, PHP, ASAH, PHP32, ACDase, SMAPME, HSD-33, HSD33, Acid ceramidase, Acylsphingosine deacylase, AC
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH1 is an acid ceramidase and key regulator of ceramide metabolism. Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulmonary insufficiency, while a complete knockout of its expression is lethal in mice. Recent studies have shown elevated levels of ASAH1 in Alzheimer's disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide. Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis. Multiple isoforms of this protein are known to exist.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-ASAH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide near the C-terminus of the human ASAH1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-ASAH1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with ASAH1 from other sources has not been determined. Multiple isoforms of ASAH1 are known to exist.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of ASAH1 antibody. Tissue: Human heart cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: ASAH1 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: ASAH1 as a red fluorescent signal.
Immunohistochemistry of ASAH1 antibody. Tissue: Human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: ASAH1 antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ASAH1 is nuclear and occasionally cytoplasmic. Staining: ASAH1 as a precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of ASAH1 antibody. Lane A: Mouse heart tissue lysate at 1 ug/mL. Lane B: Mouse heart tissue lysate at 2 ug/mL. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.6 kDa, ~52 kDa for ASAH1.