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Antibodies

DISC1 Antibody

Product Sizes
100 ug
600-401-AV9-100UG
About this Product
SKU:
600-401-AV9
Additional Names:
DISC1|DISC1 Antibody, SCZD9, C1orf136, KIAA0457, Disrupted in schizophrenia 1 protein
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Disrupted in schizophrenia 1 (DISC1) is a candidate gene for susceptibility to schizophrenia. It was discovered through chromosomal analysis of a large Scottish family whose members exhibited schizophrenia and related psychiatric disorders. Through yeast two-hybrid screening, it was discovered that DISC1 interacts with many members of the centrosome and cytoskeletal system including MAP1A and Nudel. More recently, DISC1 has been found to regulate the transport of a complex containing Nudel, the lissencephaly-1 (LIS1) protein, and 14-3-3epsilon from neuronal cell bodies to the axons by the action of the microtubule-dependent directed motor protein kinesin-1, also known as KIF5A. Decreased expression of DISC1 in neurons caused an accelerated rate of neuronal integration, resulting in aberrant morphological development, suggesting that DISC1 plays a role in dendritic development and synapse formation. DISC1 has at least four known isoforms.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-DISC1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide from near the internal region of human DISC1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-DISC1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with DISC1 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of DISC1 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: DISC1 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: DISC1 as a red fluorescent signal.
Immunocytochemistry of DISC1 antibody. Tissue: HeLa cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: DISC1 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DISC1 is nuclear and cytoplasmic. Staining: DISC1 as precipitated brown signal with hematoxylin purple counterstain.
Western Blot of DISC1 antibody. Lane A: SK-N-SH cell lysate at 0.5 ug/mL. Lane B: SK-N-SH cell lysate at 1 ug/mL. Lane C: SK-N-SH cell lysate at 2 ug/mL. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 93.6 kDa, ~93 kDa for DISC1. Other band(s): DISC1 splice variants and isoforms.