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Antibodies

CTRP3 Antibody

Product Sizes
100 ug
600-401-AP9-100UG
About this Product
SKU:
600-401-AP9
Additional Names:
C1QTNF3|CTRP3 Antibody, CORS, CORCS, CTRP3, CORS26, C1ATNF3, CORS-26, UNQ753/PRO1484, Complement C1q tumor necrosis factor-related protein 3, Collagenous repeat-containing sequence 26 kDa protein
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Multiple isoforms of human CTRP3 have been reported. It has been suggested that CTRP3 may play a role in skeletal development.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CTRP3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the internal region of human CTRP3.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-CTRP3 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of CTRP3 antibody in mouse heart cell lysate. Lane A: CTRP3 antibody in the absence of blocking peptide. Lane B: CTRP3 antibody in the presence of blocking peptide. Load: 35 ug per lane. Primary antibody: CTRP3 antibody at 1 ug/mL for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 27 kDa, 36 kDa for CTRP3. Other band(s): CTRP3 splice variants and isoforms.
Immunofluorescence Microscopy of CTRP3 antibody. Cell Type: mouse heart cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CTRP3 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CTRP3 is secreted. Staining: CTRP3 as red fluorescent signal.
Immunohistochemistry of CTRP3 antibody. Tissue: mouse heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: CTRP3 antibody at 2 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CTRP3 is secreted. Staining: CTRP3 is stained with hematoxylin purple nuclear counterstain.