Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

CARD8 Antibody

Product Sizes
100 ug
600-401-AB5-100UG
About this Product
SKU:
600-401-AB5
Additional Names:
CARD8|CARD8 Antibody, NDPP, DACAR, DAKAR, NDPP1, TUCAN, CARDINAL, KIAA0955, Caspase recruitment domain-containing protein 8, Apoptotic protein NDPP1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-kB activation.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CARD8 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
Anti-CARD8 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with CARD8 from other sources has not been determined.
Storage Conditions:
2-8[o]C
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of CARD8 antibody. Lane A: K562 at 2 ug/ml. Lane B: K562 at 4 ug/ml. Load: 35 ug per lane. Primary antibody: CARD8 antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 49 kDa, 45 kDa for CARD8. Other band(s): CARD8 splice variants and isoforms.
Immunocytochemistry of CARD8 antibody. Cell Type: K562 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: CARD8 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CARD8 is nuclear and cytoplasmic. Staining: CARD8 is stained with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of CARD8 antibody. Cell Type: K562 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CARD8 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CARD8 is nuclear and cytoplasmic. Staining: CARD8 as green fluorescent signal.