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Antibodies

Cyclin B1 phospho S126 Antibody

Product Sizes
100 ug
600-401-464-100UG
About this Product
SKU:
600-401-464
Additional Names:
CCNB1|rabbit anti-Cyclin B1 pS126 Antibody, Cyclin B1, CCNB1, CCNB, G2/mitotic-specific cyclin-B1
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
2.01 mg/ml
Extra Details:
Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 115-140 of Human Cyclin B1 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western blot using Rockland's Affinity Purified anti-Cyclin B1 pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B1 (arrowheads) in various whole cell lysates. Lane 1: Hela (cervical carcinoma) [p/n W09-000-364], Lane 2: H23 (lung carcinoma), Lane 3: Hep3b (Hepatocarcinoma), Lane 4: T98G (Glioblastoma), and Lane 5: Daudi (B cell lymphoblast) [W09-001-MQ2]. Each lane contains approximately 50 ug of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel. Transfer occurred overnight at 4°C at 15 mAmps. The membrane was blocked with 5% non-fat dry milk in TTBS (p/n B501-0500) for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature. After washes with TTBS a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-103-122) was added for 1 h at room temperature. After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min. Excess detection solution was drained off and the membrane was exposed to Kodak film X-omat blue XB-1 for about 20 sec. Other detection systems will yield similar results. Personnel Communication, Luca Cote, Temple U.