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Antibodies

Cyclin L1 Antibody

Product Sizes
500 ug
200-401-881-500UG
About this Product
SKU:
200-401-881
Additional Names:
CCNL1|rabbit anti-Cyclin L1 antibody, Cyclin-L, Cyclin L beta, Cyclin L gamma, CCNL1
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
5 mg/ml
Extra Details:
Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to an internal region near amino acids 300-375 of Human Cyclin L1a protein.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Human
Shipping Conditions:
Ambient
Specificity:
The product was purified from monospecific antiserum by Protein A chromatography. This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1b or Cyclin L2 isoforms. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L1a protein from dog, rat, mou
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland's anti-Cyclin L1a antibody was used at a 10 ug/ml to detect Cyclin L1a in a variety of tissues including breast (collagen), heart, kidney (distal tubules), liver, skeletal muscle, ovary (granulosa and oocyte), pancreas (islet), placenta (trophoblast), prostate (epithelium), skin, spleen (endothelium), stomach (chief), testes (seminiferous epithelium and leydig), thymus (Has-sal's corpuscle and lymphocytes) and uterus (glandular epithelium and stroma). Low to moderate background staining was noted. This image shows Cyclin L1a staining of human spleen. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpan Biosciences, Seattle, WA.
Western blot using Rockland's Protein A Purified anti-Cyclin L1a antibody shows detection of a band ~59 kDa corresponding to a Cyclin L1a (arrowhead) present in mouse brain whole cell lysate (p/n W10-000-T004). Approximately 35ug of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.