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Antibodies

GST Antibody Dylight[TM] 549 Conjugated

Product Sizes
100 ug
200-342-200-100UG
About this Product
SKU:
200-342-200
Additional Names:
mouse anti-GST antibody DyLight™ 549 conjugation, DyLight™549 conjugated mouse anti-GST antibody, Glutathione-S-Transferase, Anti-GST monoclonal antibody
Application:
ELISA, FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
3D4
Clonality:
Monoclonal
Conjugate:
DyLight™ 549
Concentration:
1 mg/ml
Extra Details:
Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
BALB/c mice were immunized with GST from full length Schistosoma japonicum protein. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.
Isotype:
IgG1, IgG1, kappa
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
This product was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and purified and partially purified Glutathione-S-Transferase (GST).
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Tagged Antibodies
DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
Rockland Mouse-a-GST (200-301-200 lot 24882, blue), Rabbit anti-Transferrin (109-4134 lot 3033), and Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green) DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue), and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
Properties of DyLight™ Fluorescent Dyes.