DYKDDDDK Tag (Anti-FLAG[R]) Antibody
Product Sizes
100 ug
200-301-383-100UG
About this Product
- SKU:
- 200-301-383
- Additional Names:
- mouse anti-FLAG™ tag, Enterokinase Cleavage Site (ECS), mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
- Application:
- ChIP, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- 29E4.G7
- Clonality:
- Monoclonal
- Concentration:
- 1 mg/ml
- Extra Details:
- Antibody for the detection of FLAG™ recognizes FLAG™ and is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. Antibody for the detection of FLAG™ can be used to identify fusion proteins containing the FLAG™ epitope. Antibody for the detection of FLAG™ recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Mouse
- Immunogen:
- This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
- Isotype:
- IgG2a, IgG2a, kappa
- Physical State:
- Liquid
- Reactivities:
- Label/Epitope-tag
- Shipping Conditions:
- Dry Ice
- Specificity:
- This product is an IgG fraction antibody purified from ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. The purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal anti-FLAG™ ta
- Storage Conditions:
- -20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Tagged Antibodies
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=200-301-383
