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Antibodies

RFP Antibody

Product Sizes
25 ul
200-301-379S-25UL
About this Product
SKU:
200-301-379S
Additional Names:
DsRed|mouse anti-RFP Antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Application:
Detection/Quantitation, ELISA, FISH, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
8E5.G7
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, Western blotting, immunohistochemistry, and flow cytometry. RFP Proteins are useful markers for imagining protein localization, monitoring physiological processes, and detecting transgenic expression. Rockland's anti-RFP antibody can be used to detect native RFP and RFP variants.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
Isotype:
IgG2a, IgG2a, kappa
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Western Blot of Mouse Anti-RFP antibody. Lane 1: YFP protein. Lane 2: Prestained Molecular Weight Marker. Lane 3: Reduced RFP control Protein. Lane 4: Reduced mCherry. Lane 5: GFP protein. Lane 6: Non-Reduced RFP control Protein. Lane 7: Non-Reduced mCherry. Load: 300ng per lane. Primary antibody: RFP antibody at 1:2000 in MB-070 for 3 hours at RT. Secondary antibody: HRP anti-Mouse secondary antibody at 1:10,000 in MB-070 for 60 min at RT. Substrate: TMBM-100 for 20 min. Predicted/Observed size: ~27 kDa.
ELISA results of purified Mouse anti-RFP Monoclonal Antibody tested against RFP (p/n 000-001-379). Each well was coated in duplicate with 1.0 ug of the antigen. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, anti-Mouse IgG Antibody Peroxidase Conjugated Secondary and TMB ELISA Peroxidase Substrate (p/n TMBE-1000).
Western Blot of Mouse Anti-RFP Antibody. Lane 1: Opal Prestain Molecular weight (p/n MB-210-0500). Lane 2: 50ng of RFP. Primary Antibody: Mouse Anti-RFP at 1ug/mL overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse HRP (p/n 610-403-C46) at 1:40,000 for 30mins at RT. Block: BlockOut Universal blocking buffer (p/n MB-073). Expect ~27kDa.
Transgenic model and effects of acute 4D overexpression on NSC and progenitors of the SVZDrawings of the nestinCreERT2 (Imayoshi et al, 2008), ROSA26rtTA-flox (Belteki et al, 2005), and tet4D-RFP (Nonaka-Kinoshita et al, 2013) alleles of the 4D line.From top to bottom: experimental design of 4D induction, fluorescence pictures of the SVZ of a 4D+ mouse and quantification of the proportion of RFP- (black) and RFP+ (red) progenitors among B1, C, and A cells identified with markers as indicated.From top to bottom: experimental design, fluorescence pictures of the SVZ of a 4D- (left) and 4D+ (right and insets magnified) mice, and quantification of the proportion of BrdU+ among B1, C, and A cells (identified as in B). Note that in 4D+ mice quantification was restricted to the RFP+ subpopulation (red bars).Quantification of the absolute number of B1, C, and A cells (identified as in B) in the SVZ of 4D- (white) and 4D+ (black) mice regardless of RFP expression (RFP+/-).Data information: (B-D) Mean +/- SEM; *P < 0.05 and **P < 0.01 calculated by unpaired Student's t-test; N = 3 mice, n > 423 cells for each quantification. Scale bar = 50 um (B and C) or 20 um (inset in C). Figure provided by CiteAb. Source: EMBO J, PMID: 30643018.
Characterization of the transgenic model and effect of 4D on the RMSA Fluorescence image of a sagittal section of a 4D+ brain after a 4-day treatment with doxycycline showing RFP signal confined to the SVZ and RMS (nuclei counterstained with DAPI; blue). Insets show representative images of specific brain regions (as indicated) and the olfactory epithelium. A'Phase contrast picture of the SVZ upon in situ hybridization against mRNA for RFP in a 4D+ brain treated as in (A) and sacrificed immediately after (left) or 2 days after (right) doxycycline administration. (B, C) Experimental design (top), fluorescence pictures (left with magnified insets), and quantifications (right) of BrdU incorporation in the RMS (B) or SVZ (C). (B) shows the proportion of BrdU in C (Mash1+) and A (DCX+) cells in 4D- (white) and 4D+ (red; among RFP+) mice. (C) shows the proportion of RFP- (black) and RFP+ (red) among BrdU+ cells of 4D+ mice. (A) OB, olfactory bulb; RMS, rostral migratory stream; LV, lateral ventricle; DG, dentate gyrus; OE, olfactory epithelium. (A-C) Tam, tamoxifen; Dox, doxycycline. (B, C) Mean +/- SEM; **P < 0.01; unpaired Student's t-test; N = 3 mice and n > 1,100 cells. Scale bars = 500 (A top, A'), 100 (insets A and A'), 50 (B and C), and 20 (insets B and C) um. Figure provided by CiteAb. Source: EMBO J, PMID: 30643018.