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Antibodies

Avi Epitope Tag Antibody

Product Sizes
100 ul
100-401-B21-100UL
About this Product
SKU:
100-401-B21
Additional Names:
rabbit anti-Biotin Ligase Epitope Tag Antibody, rabbit anti-Avi-tag antibody, biotin ligase, biotin operon repressor protein, biotin holoenzyme synthetase, biotin-[acetyl-CoA carboxylase] synthetase
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
90 mg/ml
Extra Details:
The peptide is also called the biotin acceptor peptide (BAP) of the biotin carboxyl carrier protein (BCCP) subunit of acetyl CoA carboxylase of E. coli.
Format:
Antiserum
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Avi Epitope Tag antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic avi-tag peptide GLNDIFEAQKIEWH (minimal BirA substrate; biotin is added to the sequence at the lysine).
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
This antiserum detects the minimal peptide substrate GLNDIFEAQKIEWH for the E. coli biotin ligase BirA. Western blot analysis of B. subtilis strains producing either (Avi-tagged) SPoIIIAH (XWB49) or (Avi-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Tagged Antibodies