Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Proteins and Peptides

Human Collagen Type VI

Product Sizes
500 ug
009-001-108-500UG
About this Product
SKU:
009-001-108
Additional Names:
COL6A1-COL6A3|Type VI collagen, collagen 6, human collagen, collagen VI
Application:
Cell-based/Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.5 M Acetic Acid
CE/IVD:
RUO
Concentration:
1 mg/ml
Extra Details:
Collagen type VI is a form of collagen primarily associated with the extracellular matrix of skeletal muscle. Collagen type VI is associated with the genes COL6A1, COL6A2, and COL6A3. Defects in collagen VI are associated with Bethlem myopathy and Ullrich congenital muscular dystrophy.
Formulation:
0.5 M Acetic Acid
Immunogen:
COL6A1-COL6A3
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Human Collagen type VI has been prepared from Human Placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type VI. Reaction with Rockland's anti-Collagen I, II, III, IV or V is negligible.
Storage Conditions:
2-8[o]C diluted.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Native
COL6 enhances lung epithelial cell wound repair.(a) Representative images of wound-healing response for 16HBE cells plated on COL6, Matrigel, or COL1. (b-c) Quantitation of wound width at 10 hr post-injury (relative to 0 hr) for 16HBE(b) and NHBE(c) plated on individual matrices, or combinations of matrices. N = 9, ** p<0.01, *** p<0.001. Fig 2. PMID: 30550606.
Dot Blot results using Rabbit Anti-Collagen VI Antibody. Sample: A) Human Collagen VI (p/n 009-001-108), B) Bovine/Human Collagen VI (p/n 001-001-108). Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng. Primary Antibody: Rabbit Anti-Collagen VI antibody (p/n 600-401-108) at 5ug/mL at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated (p/n 611-1302) 1:40,000 at RT for 30mins. Blocking: BlockOut® Universal Blocking Buffer (p/n MB-073) at RT for 30mins.
Fourier-transform infrared (FTIR) spectroscopy. Mean FTIR spectra of the five collagen types. Maximal intensity. Fig. 2. PMID: 19685340.
SDS-PAGE results of Human Collagen Type VI. Lane: 1) Opal prestain molecular weight marker (p/n MB-210-0500), 2) blank, 3) Human Collagen Type VI (p/n 009-001-108) [10ug]. Coomassie stained.
Western blot results using Rabbit Anti-Collagen Type VI Antibody. Lane: 1) Human Collagen Type VI (p/n 009-001-108) reduced and boiled, 3) Human Collagen Type VI reduced and not boiled,5) Opal pre-stained molecular weight marker (p/n MB-210-0500), 7) Human Collagen Type VI not reduced and boiled, 9) Human Collagen Type VI not reduced and not boiled. Lanes 2, 4, 6, 8 blank. Load: 10ug. Primary Antibody: Rabbit Anti-Collagen Type VI Antibody (p/n 600-401-108) 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP conjugated (p/n 611-1302) 1:40,000 at RT for 30mins. Blocking: BlockOut® Universal Blocking Buffer (p/n MB-073) at RT for 1hr.