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Bovine Albumin Rhodamine

Product Sizes
1 mg
001-0033-1MG
About this Product
SKU:
001-0033
Additional Names:
Bovine Albumin Rhodamine conjugated, Bovine Albumin TRITC conjugated, BSA Rhodamine conjugated, BSA TRITC conjugated
Application:
Cell-based/Functional Assay, Dot Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
TRITC
Concentration:
1 mg/ml
Extra Details:
Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Albumin and anti-Bovine Serum.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
CP1 labels the protein endocytic pathway of the cytostome/cytopharynx complex. SR-SIM IFAs of epimastigotes after endocytosis of Transferrin (A, green), IgG (B, red), and BSA (C, red), shows labeling of the SPC endocytic structure by CP1-Ty. (D) Strategy for evaluating amastigote endocytosis of host-cell cytosolic protein labeled by CFSE. (E) SR-SIM IFA of an amastigote that has endocytosed CFSE labeled host cytosolic protein, coinciding with the SPC labeled by CP1-Ty. Scale bars: 2 um. Figure 2. PMID: 32010635.
Overexpressed CP1-mNeon-Ty Localizes to the SPC. (A) Vector map of the CP1-pTREX overexpression plasmid with a common detrimental mutation of the neomycin cassette restored (i). This fixed neomycin cassette allows for harsh G418 selection of epimastigotes at up to 2,000 ug/mL. (B) SR-SIM microscopy of a CP1-mNeon epimastigote after the endocytosis of BSA-Rhodamine shows that BSA and CP1-mNeon label the same endocytic pathway. (C) Treating epimastigotes with cytochalasin B during the assay prevented endocytosis via the SPC, causing BSA to strongly label the pre-oral ridge in front of the cytostome (arrow). (D) SR-SIM of amastigotes expressing CP1-mNeon-Ty shows SPC labeling. (E) SR-SIM images of epimastigotes expressing CP1-mNeon-Ty, showing SPC labeling. Labeling at 4°C with Concanavalin A - Rhodamine was used to identify the entrance to the SPC (arrow). Scale bars: 2 um. Figure 3. PMID: 32010635.