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Antibodies

Rabbit Anti-Glucocorticoid Receptor Antibody [JF0952], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
ET1702-11-ET1702-11
About this Product
SKU:
ET1702-11
Application:
WB, FC
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JF0952
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
Zebrafish tissue lysate, A549 cell lysate, HepG2 cell lysate, hybrid fish (crucian-carp) heart tissu
Format:
Liquid
Host:
Rabbit
Immunogen:
Recombinant protein within Human Glucocorticoid Receptor aa 2-220 / 777.
Isotype:
IgG
Molecular Weight:
86 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat, Zebrafish
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Glucocorticoid Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Zebrafish tissue lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate
Western blot analysis of Glucocorticoid Receptor on hybrid fish (crucian-carp) heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
FlowCytometric analysis of Glucocorticoid Receptor was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-11, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).