Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Rabbit Anti-Cytochrome C Antibody [SC59-01], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
ET1610-16-ET1610-16
About this Product
SKU:
ET1610-16
Application:
WB, IF-Cell, IHC-Paraffin, IP
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SC59-01
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
mouse kidney tissue lysates, rat kidney tissue lysates, Hela, human liver tissue, human kidney tissu
Format:
Liquid
Host:
Rabbit
Immunogen:
Synthetic peptide within human Cytochrome C aa 10-50.
Isotype:
IgG
Molecular Weight:
12 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Cytochrome C on different lysates using anti-Cytochrome C antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse kidney tissue lysates Lane 2: Rat kidney tissue lysates
ICC staining of Cytochrome C in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.