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Antibodies

Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody

Product Sizes
100 ul
SP1641-100UL
About this Product
SKU:
SP1641
Additional Names:
SK1, SPHK1, SPK1
Application:
Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser-225 - Phosphorylated
Extra Details:
Our Sphingosine Kinase 1 (Ser-225) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Sphingosine Kinase 1 (Ser-225) and is antigen affinity purified. It is great for use in WB.
Format:
Antigen Affinity Purified
Gene Details:
SPHK1
Host:
Rabbit
Immunogen:
Phospho-SK1 (Ser-225) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 225 in human SK1. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and is not homologous to sequences in SK2. The conserved site in rat and mouse SK1 is at serine 224.
Isotype:
IgG
Molecular Weight:
42
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
This antibody was cross-adsorbed to non-specific phosphoserine and unphosphorylated SK1 (Ser-225) peptide before affinity purification using phospho-SK1 (Ser-225) peptide. The purified antibody detects a 47 kDa* full-length recombinant human SK1 protein phosphorylated with ERK2.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Western blot image of recombinant his-tagged human SK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-SK1 (Central Region) (SP1621; lanes 1-3) and anti-SK1 (Ser-225) (SP1641; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-SK1 (Ser-225) peptide (SX1645; lanes 2 & 5), or unphosphorylated SK1 (Ser-225) peptide (SX1625; lanes 3 & 6).
Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK1 (Central region) SP1621 (lanes 1 & 2) or anti-SK1 (Ser-225) SP1641 (lanes 3 & 4).
The protein expression levels of DGKA Alpha, SphK1 (cat. SP5421, 1:1000) and p-SphK1(Ser225) (cat. SP1641) in AML cells after ritanserin treatment for 48 h. Image from publication CC-BY-4.0. PMID: 37392305
In separate experiments, human pulmonary artery ECs were treated with a pharmacologic SphK1 inhibitor, PF-543 (10 uM), 1 h prior to irradiation, followed by Western blotting for p-SphK1 (cat. SP1641), SphK1, and UCHL1. Representative blots are shown. Image from publication CC-BY-4.0. PMID: 37830619