A431 Cell Nuclear Extract
Product Sizes
A431 Cell Nuclear Extract
£ POA
W09-001-A60
About this Product
- SKU:
- W09-001-A60
- Additional Names:
- A431 Cell Nuclear Extract, A431 Nuclear Lysate, A431 Lysate Nuclear Extract
- Application:
- Western Blot
- Buffer:
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
- Concentration:
- 1 mg/ml
- Physical State:
- Liquid (sterile filtered)
- Shipping Conditions:
- Dry Ice
- Specificity:
- The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). The lysate was prepared by first washing the cells in PBS. Washed cells were then incubated on ice in lysis buffer containing 10 mM HEPES, 60 mM KCl, 1.0 mM EDTA, 0.075% (v/v) NP40 and 1.0 mM DTT, pH 7.6. Protein integrity is ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspa
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Lysates
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=W09-001-A60