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Assay Kits & ELISAs

CellCountEZ[TM] Cell Survival Assay Kit

Product Sizes
1 kit
KLD-001-1KIT
About this Product
SKU:
KLD-001
Additional Names:
Toxicity Proliferation and Survival Assay, cell proliferation and cell survival assay, cell viability kit, cell survival kit, cellular viability, cellular survival, HEDS assay, hydroxyethyl disulfide assay, quantifying cell growth and survival, cellular Drug toxicity
Application:
Bioactivity, Cell-based/Functional Assay
CE/IVD:
RUO
Extra Details:
CellCountEZ™ Cell Survival Assay Kit is a tissue culture media-based assay that can measure metabolically active live cells and quantify cell death caused by radiation, chemotherapeutics or toxins. CellCountEZ™ is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). CellCountEZ™ measures ME in the extracellular medium without the need for cellular lysis and extraction methods, this preserves the ability to perform other cellular tests in the same culture system. CellCountEZ™ is readily soluble, membrane permeable and converted by live cells intracellularly before transport into the extracellular culture media. CellCountEZ™ offers many advantages that make it superior to common existing methods for quantifying cell growth and survival.
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Supplier:
Rockland Inc
Type:
Assay Kits: Cell Viability
Schematic representation of the various pathways involved in the cellular interactions of HEDS. HEDS reacts spontaneously with glutathione (GSH) or in a reaction catalyzed by glutathione-S-transferase (GST) to produce mixed disulfide (MESSG) of GSH and mercaptoethanol (ME) (Pathway 1). The mixed disulfide MESSG reacts with GSH and produces ME and GSSG by the catalytic action of glutaredoxin (GRX) (Pathway 2). The glutathione disulfide GSSG reacts with NADPH and produces GSH by the catalytic action of glutathione reductase (GRase) (Pathway 3). The conversion of GSSG to GSH i.e. GSH recycling requires NADPH recycling (NADP+ NADPH) by oxidative pentose phosphate cycle (OPPC) (Pathway 4).
CellCountEZ™ Assay for human colon cancer cells HCT116 in a 96 well plate. This assay gives a linear response for cells (0; 1,000; 5,000; 10,000; 20,000; 40,000) plated in 100 ul growth medium with up to 15% fetal bovine serum in a 96-well plate and measured 20 hours after plating. The background (no cells) O.D. is 0.391, 0.381, 0.368 for expt 1,2, and 3 repectively. The r2 values close to 1 demonstrate the dynamic range up to 40,000 cells. y=0.87x-0.008 r2=1.000. y=0.083x+0.045 r2=0.997. y=0.088x+0.052 r2=0.997.
CellCountEZ™ Cell Survival Assay Kit is a tissue culture media-based assay that can measure metabolically active live cells and quantify cell death caused by radiation, chemotherapeutics or toxins. CellCountEZ™ Assay after 24 hours treament with 0-50 uM of Cisplatin on human colon cancer cells HCT116 in a 96 well plate.
CellCountEZ™ Assay after 3 days of treament with 0-1000 uM of hydrogen peroxide on human colon cancer cells HCT116 in a 96 well plate. Drug toxicity could be measured for up to 4 days after treatment with drugs using this assay.
CellCountEZ™ activity from the mean of 72 samples in a 96 well. CellCountEZ™ Assay after 0-8 doses of radiation on human colon cancer cells HCT116. CellCountEZ™ measures mercaptoethanol in the extracellular medium without the need for cellular lysis and extraction methods.