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Antibodies

BORA Antibody

Product Sizes
100 ug
600-401-Z84-100UG
About this Product
SKU:
600-401-Z84
Additional Names:
BORA|Bora Antibody, C13orf34, Protein aurora borealis, HsBora
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Bora antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the C-terminus of human Bora.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-Bora Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Bora from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of Bora antibody. Tissue: Mouse brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Bora antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Bora is cytosolic. Staining: Bora as red fluorescent signal.
Western Blot of Bora antibody. Lane 1: Mouse brain tissue lysate with Bora antibody at 1 ug/mL. Lane 2: Mouse brain tissue lysate with Bora antibody at 2 ug/mL. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61 kDa, 79 kDa for Bora. Other band(s): Bora splice variants and isoforms.
Immunohistochemistry of Bora antibody. Tissue: mouse brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Bora antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Bora is cytosolic. Staining: Bora as precipitated brown signal with purple counterstain.