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Antibodies

ACE2 Antibody

Product Sizes
100 ug
600-401-X59-100UG
About this Product
SKU:
600-401-X59
Additional Names:
ACE2|ACE2 Antibody, ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-ACE2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the N terminus of human ACE2.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Anti-ACE2 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Predicted species reactivity based on immunogen sequence: Bovine: (86%). Anti-ACE2 has no cross response to ACE1.
Storage Conditions:
2-8[o]C
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of ACE2 antibody. Cell Type: human kidney cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: ACE2 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ACE2 is located in the cell membrane and cytoplasm. Staining: ACE2 as red fluorescent signal.
Western Blot of Rabbit Anti-ACE2 Antibody. Lane 1: Human Kidney Lysate. Lane 2: Human Spleen Lysate. Lane 3: Human Testis Lysate. Lane 4: Human Breast Lysate. Lane 5: Human Intestine Lysate. Load: 15ug of lysates per lane. Primary Antibody: ACE2 (600-401-X58, 600-401-G71, 600-401-X59) at 2ug/mL and Beta Actin at 1ug/mL for 1hr at RT in 5% BLOTTO/TBST. Secondary Antibody: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted MW: ~93kDa. Observed MW: ~130kDa.
Immunofluorescence of Rabbit Anti-ACE2 Antibody. Tissue: Human Testis Tissue. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Western Blot of ACE2 antibody. Load: human kidney lysate 35 ug per lane. Primary antibody: ACE2 antibody at 1ug/ml for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 92 kDa, 95 kDa for ACE2. Other band(s): ACE2 splice variants and isoforms.
Immunofluorescence of Rabbit Anti-ACE2 Antibody. Tissue: Human Lung Tissue. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).