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Antibodies

p53 K305 Ac Antibody

Product Sizes
100 ug
600-401-GW2-100UG
About this Product
SKU:
600-401-GW2
Additional Names:
rabbit anti-p53 Ac-Lys305 antibody, rabbit anti-p53 K305ac antibody, cellular tumor antigen p53, tumor suppressor p53, phosphoprotein p53, Antigen NY-CO-13, TP53|TP53
Application:
Dot Blot, ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
TP53 (tumor suppressor gene p53) is one of the most well-studied genes that suppresses tumor formation and renders protection against DNA damage by inducing cell cycle arrest, DNA repair, or apoptosis. TP53 signaling is triggered through numerous cellular events ranging from DNA damage to hypoxia, stress and a plethora of other causes. Upon activation, p53 acts as zinc-containing transcriptional regulator and initiates a cascade of events that determines the cellular outcome including cell cycle arrest, apoptosis, senescence, DNA repair, development, differentiation and tissue homeostasis. Cell cycle arrest is induced by p53 via trans-activating genes such as p21 (CDK-inhibitor 1, cyclin dependent kinase) and others. Interestingly, p53 itself is capable of triggering cellular responses (survival or induced cell death) as well. Mutations or deletions in the TP53 gene are present in nearly 50% of human cancers, and primarily results in impaired tumor suppressor function. Anti-p53 (ac Lys305) antibody is ideal for researchers interested in developmental biology, cell growth and cancer research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-p53 K305 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 305 of human p53.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-p53 K305 Ac antibody is directed against human p53 protein. p53 K305 Ac Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis used to suggest reactivity with this protein from human based on 100% homology for the immunizing sequence.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Dot Blot of Rabbit Anti-p53 305KAc Antibody. Lane 1: Unmodified p53 peptide. Lane 2: 305KAc p53 peptide. Loaded: 100, 10, 1 picomoles of peptide. Primary Antibody: Left Membrane: 1.0 ug/ml of purified antibody; Middle Membrane: 0.5 ug/ml; Right Membrane: 0.25 ug/ml. Blocking: BLOTTO Blocking Buffer (p/n B501-0500) for 30min at RT. Secondary Antibody: Gt-A-Rb IgG HRP (p/n 611-103-122) at 1:40,000.
Western Blot of RABBIT Anti-p53 (ac Lys305) Antibody. Lane 1: Ladder Opal PreStained (p/n MB-210-0500). Lane 2: MCF-7 (p/n W09-000-360). Lane 3: MCF-7 Nuclear Extract (p/n W09-001-A85). Lane 4: Jurkat Lysate (p/n W09-001-370). Lane 5: HeLa Whole Cell Lysate (p/n W09-000-364). Lane 6: A431 Whole Cell Lysate (p/n W09-000-361). Loaded at 10ug. Blocking: BlockOut Buffer (p/n MB-073) for 30 min at RT. Primary Antibody: Rb anti-p53 K305 Ac at 1ug/mL for 4°C overnight. Secondary Antibody: Gt-a-Rb HRP (p/n 611-103-122) at 1:70,000 in MB-073 for 30 min at RT. Expected: ~53 kDa.