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Antibodies

AKT Antibody

Product Sizes
25 ul
200-301-401S-25UL
About this Product
SKU:
200-301-401S
Additional Names:
AKT1, AKT2, AKT3|mouse anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT2, AKT3
Application:
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
14E5.16C8.25F6
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Anti-AKT Antibody was produced by repeated immunizations in mice with a synthetic peptides corresponding to residues internal to the human AKT1, AKT2, and AKT3 proteins.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Non-human Primate, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-AKT Antibody was purified by Protein A chromatography. This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
ELISA of Mouse Anti-AKT Antibody. Antigen: HCT-116 cell line (p/n W09-001-GM4). Coating amount: Confluent in the 96 well plate. Primary antibody: AKT (top) or GAPDH (bottom) antibody at 2 ug/mL. Dilution series: Primary and Secondary Antibodies 2-fold. Mid-point concentration: N/A. Secondary antibody: DyLight™ 680 donkey secondary antibody and DyLight™ 800 goat secondary antibody starting at 1:1,000. Substrate: None.
Western Blot of Mouse anti-AKT antibody. Lane 1: NIH/3T3 cell lysates (p/n W10-000-358). Lane 2: PDGF stimulated NIH/3T3 cell lysates (p/n W10-001-377). Load: 10ug per lane. Primary antibody: Anti-AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: Goat-anti-Mouse IgG HRP conjugated (p/n 610-103-121) was used at a 1:40,000 dilution for 1hr at 2-8°C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500) in TBS for 2hr at RT. Observed size: ~56 kDa for AKT.
Western Blot of Mouse anti-AKT Monoclonal Antibody. Lane 1: His-AKT1 Recombinant Protein. Lane 2: His-AKT2 Recombinant Protein. Lane 3: His-AKT3 Recombinant Protein. Load: 50 ng per lane. Primary antibody: Mouse anti-AKT Monoclonal Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 54-56 kDa, 54-56 kDa for AKT1, AKT2, AKT3.
SDS PAGE of Mouse anti-AKT Monoclonal Antibody. Lane 1: Reduced Mouse anti-AKT Monoclonal Antibody. Lane M: 3 uL Opal Prestained Marker (p/n MB-210-0500). Lane 2: Non-Reduced Mouse anti-AKT Monoclonal Antibody. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 160kDa/Observed at 245 kDa; Reduced at 55, 25 kDa. Non-reduced migrates slightly higher.