Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight[R] 649
- SKU:
- DL-1208
- Additional Names:
- Lectins & Glycobiology Reagents [Lectins ], Bandeiraea Lectin I, Isolectin B4, GSL I-B4, Galactose binding lectin
- Application:
- Immunofluorescence
- Conjugate:
- DyLight 649
- Extra Details:
GSL I is a family of glycoproteins with molecular weights of approximately 114kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.
GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.  This “Bâ€-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues.
DyLight[R]Â 649 GSL I-B4Â isolectin is produced by using special conjugation procedures to incorporate DyLight[R]Â 649 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.
- Excitation maximum: Â 646 nm
- emission maximum: Â 674 nm
- Color: Â Red
Inhibiting Sugar: 500 mM galactose or 100 mM raffinose
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8 [o]C
- Supplier:
- Vector Laboratories
- Manufacturer's Data Sheet:dylight-649-gsl-i-isolectin-b4