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Antibodies

GABARAP Antibody: Biotin

Product Sizes
100 ug
SPC-620D-BI-100UG
About this Product
SKU:
SPC-620D-BI
Additional Names:
ATG8A Antibody, GABARAP A Antibody, GABA(A) Receptor Associated Protein Antibody, MM46 Antibody
Application:
Immunocytochemistry, Immunofluorescence, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
Gamma-aminobutyric acid receptor-associated protein, or GABARAP, are lighand gated chloride channels that mediate inhibitory neurotransmission. It clusters neurotransmitter receptors by mediating its interaction with the cytoskeleton (1).
Host:
Rabbit
Immunogen:
Synthetic peptide from the N-terminal of human GABARAP
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Specificity:
Detects ~14-16kDa.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Polyclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Endomembrane System, Cytoplasm, Golgi Apparatus Membrane, Cytoplasmic Vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAP Antibody (D) Composite.
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620). Lane 1: MW Ladder. Lane 2: Human 293T (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (Complex).
Western blot analysis of Mouse Kidney showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (Complex).