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Antibodies

EAAC1 Antibody, Clone N180/41: APC

Product Sizes
100 ug
SMC-406D-APC-100UG
About this Product
SKU:
SMC-406D-APC
Additional Names:
Excitatory amino-acid carrier 1 Antibody, excitatory amino acid transporter 3 Antibody, EAAT3 Antibody, Neuronal and epithelial glutamate transporter Antibody, Sodium-dependent glutamate/aspartate transporter 3 Antibody, solute carrier family 1 member 1 and Slc1a1 Antibody
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
N180/41 (Formerly sold as S180-41)
Clonality:
Monoclonal
Conjugate:
APC
Concentration:
1 mg/ml
Extra Details:
SLC1A1/EAAT3/EAAC1, a member of this transporter family, is expressed throughout the body, particularly in brain, intestine, liver, heart and kidney. Functional defects of the SLC1A1 gene are found in patients of dicarboxylicamino aciduria.
Host:
Mouse
Immunogen:
Fusion protein amino acids 1-524 (full length) of human EAAC1
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~60kDa.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone N180/41 (SMC-406). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody (SMC-406) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) EAAC1 Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone N180/41 (SMC-406). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody (SMC-406) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) EAAC1 Antibody. (D) Composite.
Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone N180/41 (SMC-406). Primary Antibody: Mouse Anti-EAAT3 Monoclonal Antibody (SMC-406) at 1:1000.