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Proteins and Peptides

12 Epitope Tag Control Lysate (MBP)

Product Sizes
100 ul
MB-301-0100-100UL
About this Product
SKU:
MB-301-0100
Additional Names:
Flag-tag marker, MBP-tag marker, HA-tag marker, 6XHIS-tag marker, T7-tag marker, cMyc-tag marker, V5-tag marker, S-tag marker, HSV-tag marker, VSV-tag marker, Glu-Glu-tag marker, E-tag marker, Lysate marker, Protein Marker, Loading control, epitope tag marker, positive control marker
Application:
SDS-PAGE, Western Blot
Buffer:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue)
CE/IVD:
RUO
Concentration:
1 mg/ml
Extra Details:
Epitope tags are short peptide sequences easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
Formulation:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Physical State:
Liquid
Shipping Conditions:
Dry Ice
Specificity:
Control lysate made in E. coli for Western blotting expresses 12 different epitope tags.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Lysates
Western blot of 12-Epitope Tag Protein Marker lysate. The blot shows intense signal corresponding to the 12-Epitope Tag Protein Marker. The recommended load is 1 ul of lysate per lane for SDS-PAGE followed by western blotting. The membrane was probed with primary antibody at a 1:1,000 dilution in 5% BLOTTO in TBST overnight at 4°C.