TrueBlot[R] Immunoprecipitation and Western Blot Kit for 6X HIS Epitope Tag
Product Sizes
1 kit
88-8887-382-1KIT
About this Product
- SKU:
- 88-8887-382
- Additional Names:
- HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, 6X HIS Western Blot, Western Blotting Kit, Chemiluminescent 6X HIS Kit, Immunoblotting Kit, HRP Anti 6X HIS, 6X HIS Epitope Tag, 6X HIS, IP, H-H-H-H-H-H Tag, Epitope Tag
- Application:
- Immunoprecipitation, Western Blot
- Buffer:
- 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- 33D10.D2.G8
- Clonality:
- Monoclonal
- Conjugate:
- HRP
- Extra Details:
- TrueBlot® Immunoprecipitation and Western Blot Kit for 6X HIS Epitope Tag allows for the immunoprecipitation and detection of 6X HIS-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-6X HIS. Detection of the membrane bound antibody-antigen complex is achieved by the addition of Mouse IgG TrueBlot® ULTRA HRP. The TrueBlot® Immunoprecipitation and Western Blot Kit for 6X HIS Epitope Tag contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples containing a 6X HIS Epitope Tag using TrueBlot monoclonal secondary antibody in conjunction with Rockland's Anti-6X HIS (MOUSE) Monoclonal Antibody. Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated Anti-Mouse IgG monoclonal secondary antibody which enables detection of immunoblotted target protein bands, without hindrance from interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your conventional HRP Anti-Mouse IgG immunoblotting secondary antibody. It is easy to generate publication-quality IP/WB data with Mouse IgG TrueBlot® ULTRA. Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
- Formulation:
- 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Isotype:
- IgG
- Physical State:
- Liquid
- Reactivities:
- Mouse
- Shipping Conditions:
- Blue Ice
- Specificity:
- This TrueBlot® Immunoprecipitation and Western Blot Kit for 6X HIS Epitope Tag allows for the detection of 6X HIS-tagged recombinant protein present in cell lysates provided by the user. Mouse TrueBlot® ULTRA Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against ant
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Assay Kits: Western Blot
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=88-8887-382
