Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Collagen Type I Antibody Biotin Conjugated

Product Sizes
100 ug
600-406-103-100UG
About this Product
SKU:
600-406-103
Additional Names:
COL1A1|rabbit anti-collagen type I antibody biotin conjugation, biotin conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1
Application:
Detection/Quantitation, Dot Blot, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Collagen Type I from human and bovine placenta.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Ambient
Specificity:
This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negl
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Flow Cytometry of Anti-Collagen Type I Biotin Conjugated Antibody (600-406-103). Cells: mouse lung. Stimulation: none. Primary antibody: biotin conjugated anti-collagen type I antibody. Secondary antibody: PE-conjugated CD45 and PE-conjugated anti-collagen type I secondary antibody.
Flow Cytometry of Rabbit Anti-Collagen 1 Antibody. Cells: primary adult human dermal fibroblast cells. Stimulation: none. Primary antibody: Biotin-Conjugated Collagen 1 antibody (600-406-103) at 5ug/mL for 45 min at 4°C. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1:500 for 15 min at RT. Courtesy of D. Figueroa NIH.
Flow cytometry of Anti-Collagen Type I Antibody Biotin Conjugated. Expanded CD11chiCD123-CD14- cells are fibrocytes that mediate angiogenesis. (A) Using the same gating strategy as shown in Figure 1A, CD11chiCD123-CD14- cells from a representative subject sample were analyzed for cell surface phenotype. The shaded areas represent background fluorescence on the designated population as indicated by FMO controls. This is representative of more than 10 experiments. Figure 2. PMID: 23757729.
Flow Cytometry of Anti-Collagen Type I Antibody Biotin Conjugated. IL-4 induces monocytes to differentiate into CD14- fibrocytes that are readily distinguished from CD14+ macrophages in the same culture. (B) Cell surface phenotype of IL-4-differentiated adherent cells identifies 2 subsets based on CD14 expression, which further shows differential expression of collagen and TSLPR. FMO controls on gated CD14+ vs CD14- populations are shown by shaded gray histograms. This is representative of more than 5 experiments from 5 separate healthy donors. Figure 5. PMID: 23757729.