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Antibodies

ADAP Antibody

Product Sizes
100 ug
600-401-X71-100UG
About this Product
SKU:
600-401-X71
Additional Names:
ADAP Antibody, ADAP, PRO0823, SLAP-130, SLAP130, FYN-binding protein, Adhesion and degranulation promoting adaptor protein, FYB-120/130, p120/p130, FYN-T-binding protein, SLP-76-associated phosphoprotein|FYB1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-ADAP antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the N-terminus of human ADAP.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-ADAP Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with ADAP from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of ADAP antibody. Lane A: K562 cell lysate at 0.5 ug/mL. Lane B: K562 cell lysate at 1 ug/mL. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85.3 kDa, ~119 kDa for ADAP.
Immunocytochemistry of ADAP antibody. Tissue: K562 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: ADAP antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ADAP is nuclear and cytoplasmic. Staining: ADAP as precipitated brown signal with hematoxylin purple counterstain.
Immunofluorescence Microscopy of ADAP antibody. Tissue: K562 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: K562 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: ADAP as a red fluorescent signal.