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Antibodies

Cas 9 Antibody

Product Sizes
100 ug
600-401-GK0-100UG
About this Product
SKU:
600-401-GK0
Additional Names:
cas9|rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody
Application:
Detection/Quantitation, ELISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Bacteria
Shipping Conditions:
Dry Ice
Specificity:
Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15ug per lane. Primary Antibody cas9 used at 1ug/mL using MB-070 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.
Immunofluorescence of Rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1ug/mL. Secondary: Donkey anti-Rabbit at 1ug/mL, staining seen in red. Bottom images show GFP stain overlay.
Western Blot results of Rabbit Anti-Cas 9 Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: HEK/293 Cas9 Over Expressing WCL. Lane 3: HEK/293 non Transfected WCL. Load: 10ul. Primary Antibody: Rabbit Anti-Cas 9 Antibody at 1ug/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30min at RT. Blocking: BlockOut (p/n MB-073) for 30 min at RT.