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Antibodies

14-3-3 phospho S58 Antibody

Product Sizes
100 ul
600-401-D02-100UL
About this Product
SKU:
600-401-D02
Additional Names:
14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha N-terminally processed, Ywhab|Ywhab
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.01 M HEPES, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
14-3-3 pS58 antibody detects 14-3-3 protein phosphorylated at S58. 14-3-3 is a member of a family of highly conserved proteins that appear to have multiple roles in cell signaling. 14-3-3 is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders. 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms. 14-3-3 affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation. Anti-14-3-3 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Formulation:
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Host:
Rabbit
Immunogen:
Anti-14-3-3 pS58 antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-14-3-3 pS58 antibody is directed against rat 14-3-3 protein phosphorylated at S58. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a singl
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit Anti-14-3-3 pS58 Antibody. Lane 1: rat brainstem lysate. Lane 2: rat brainstem lysate blocked by the phosphopeptide. Load: 10 ug per lane. Primary antibody: 14-3-3 pS58 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~29kDa, ~29kDa for 14-3-3 protein phosphorylated at Ser58. Other band(s): none.