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Antibodies

DNase II Antibody

Product Sizes
100 ug
600-401-AW6-100UG
About this Product
SKU:
600-401-AW6
Additional Names:
DNase II Antibody, DNL, DNL2, DNASE2A, Deoxyribonuclease-2-alpha, Acid DNase, DNase II alpha|DNASE2
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-DNase II antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid peptide near the C-terminus of human DNase II.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-DNase II Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with DNase II from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of DNase II antibody. Lane 1: Human spleen tissue lysate. Lane 2: Human spleen tissue lysate in the presence of blocking peptide. Load: 35 ug per lane. Primary antibody: DNase II antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 39.6 kDa, 45 kDa for DNase II.
Immunohistochemistry of DNase II antibody. Tissue: Human spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: DNase II antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DNase II is lysosomal and occasionally extracellular. Staining: DNase II as precipitated light brown signal with hematoxylin blue nuclear counterstain.