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Antibodies

CIKS Antibody

Product Sizes
100 ug
600-401-AK8-100UG
About this Product
SKU:
600-401-AK8
Additional Names:
CIKS Antibody, ACT1, CIKS, C6orf2, C6orf4, C6orf5, C6orf6, CANDF8, PSORS13, Adapter protein CIKS, Nuclear factor NF-kappa-B activator 1, ACT1|TRAF3IP2
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB associates with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase (IKK) complex that contains IKKa, IKKb, and IKKgamma. A novel molecule that associates with and activates IKK was recently identified and designated CIKS (for connection to IKK and SAPK/JNK) and Act1 (for NF-kB activator 1). CIKS directly interacts with IKKgamma. CIKS/Act1 also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). These results indicate that CIKS/Act1 is involved in the inflammation and stress responses. CIKS/Act1 is ubiquitously expressed in human tissues.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CIKS antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid peptide near the C-terminus of human CIKS.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-CIKS Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with CIKS from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of CIKS antibody. Lane 1: Human placenta tissue lysate. Load: 35 ug per lane. Primary antibody: CIKS antibody at 1 ug/mL for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 64.7 kDa, 60 kDa for CIKS.
Immunofluorescence Microscopy of CIKS antibody. Tissue: Human lung cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CIKS antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: CIKS as red fluorescent signal.
Immunohistochemistry of CIKS antibody. Tissue: Human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: CIKS antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: CIKS as precipitated brown signal with hematoxylin blue nuclear counterstain.