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Antibodies

Cbl Antibody

Product Sizes
100 ug
600-401-AE8-100UG
About this Product
SKU:
600-401-AE8
Additional Names:
CBL|Cbl Antibody, CBL2, NSLL, C-CBL, RNF55, FRA11B, CBL2, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
The mammalian cbl family of ubiquitin ligases consists of three homologs known as cbl (also known as c-Cbl), Cbl-B, and Cbl-3 which share highly conserved a tyrosine-kinase-binding domain, linker and RING finger domain in their amino-terminal halves. Similar to other E3 ubiquitin ligases, Cbl catalyzes the transfer of ubiquitin from an E2 or Ubc (ubiquitin-conjugating) enzyme to the e-amino group of a lysine residue of the substrate protein. Cbl acts to negatively regulate many types of cell-surface receptors, including the Syk protein tyrosine kinase family. Cbl is thought to be involved in T- and B-cell signaling, in addition to thymus development. Of the three known homologs in the cbl family, cbl antibody reacts specifically with cbl. Multiple isoforms of cbl have been reported.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Cbl antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from near the C-terminus of human cbl.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-Cbl Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Cbl from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of cbl antibody in Daudi cell lysate. Lane 1: cbl antibody at 0.5 ug/mL. Lane 2: cbl antibody at 1 ug/mL. Lane 2: cbl antibody at 2 ug/mL. Load: 35 ug per lane. Primary antibody: cbl antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 97 kDa, 118 kDa for cbl. Other band(s): cbl splice variants and isoforms.
Immunofluorescence Microscopy of cbl antibody. Tissue: human lymph node tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: cbl antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: cbl is located in the cell membrane and cytoplasm. Staining: cbl as red fluorescent signal.