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Antibodies

Caspase-1 Antibody

Product Sizes
100 ug
600-401-AC5-100UG
About this Product
SKU:
600-401-AC5
Additional Names:
CASP1|Caspase-1 Antibody, ICE, P45, IL1BC, IL1BCE, Caspase-1, Interleukin-1 beta convertase, CASP-1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1b and IL-18. Caspase-1 was initially identified as an IL-1b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Caspase-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the internal region of human Caspase-1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-Caspase-1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Depending on cell lines or tissues used, other cleavage products may be observed.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Caspase-1 antibody in HeLa cell lysate. Lane A: Caspase-1 antibody at 0.5 ug/mL. Lane B: Caspase-1 antibody at 1 ug/mL. Lane B: Caspase-1 antibody at 2 ug/mL. Load: 35 ug per lane. Primary antibody: Caspase-1 antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45 kDa, 49 kDa for Caspase-1. Other band(s): Caspase-1 splice variants and isoforms.
Immunofluorescence Microscopy of Caspase-1 antibody. Cell Type: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Caspase-1 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-1 is cytoplasmic. Staining: Caspase-1 as red fluorescent signal.
Immunohistochemistry of Caspase-1 antibody. Tissue: human spleen tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Caspase-1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-1 is cytoplasmic. Staining: Caspase-1 is stained with hematoxylin purple nuclear counterstain.