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Antibodies

Cenexin-1 phospho S796 Antibody

Product Sizes
100 ug
600-401-A47-100UG
About this Product
SKU:
600-401-A47
Additional Names:
ODF2|rabbit anti-Cenexin-1 pS796 Antibody, ODF84 antibody, Outer dense fiber of sperm tail 2, Cenexin1 variant 1 antibody, KKT 4 antibody, ODF2, Cenexin 1, Cenexin-1, Outer dense fiber protein 2
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Cenexin-1, also known as ODF2 and outer dense fiber of sperm tails 2, are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human cenexin-1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Non-human Primate
Shipping Conditions:
Dry Ice
Specificity:
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human Cenexin-1 protein phosphorylated at S796. A BLAST analysis was used to suggest cross-reactivity with Cenexin-1 from human, chimpanzee and macaque based on 100
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit Anti-Cenexin-1 pS796. Lane 1: MW. Lane 2: Human Semen Lysate. Lane 3: MCF7 WCL (p/n W09-000-360). Lane 4: Molt 4 WCL (p/n W09-001-GK2). Lane 5: HeLa WCL (p/n W09-000-364). Load: 10ug per lane. Blocking: (p/n MB-073). Primary Antibody: Anti-Cenexin-1 pS796 at 1ug/mL overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) 1:40,000 diluted in (p/n MB-073) for 30 minutes at RT. Expect: 93kDa.
ELISA of Rabbit Anti-Cenexin-1 pS796 Antibody. Antigen: BSA conjugates of Cenexin-1 pS796 (purple) and S796 (blue). Coating amount: 0.1 ug per well. Primary antibody: Cenexin-1 pS796 Lot 35048 at 5 ug/mL. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody at 1:8,500. Substrate: TMB (p/n TMBE-0100).