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Antibodies

Caspase-2 Antibody

Product Sizes
100 ug
600-401-412-100UG
About this Product
SKU:
600-401-412
Additional Names:
Casp2|rabbit anti-Caspase-2 Antibody, Apoptosis related cysteine peptidase antibody, CASP-2, CASP 2 antibody, Casp 2L Pro antibody, CASP2 antibody, Caspase 2 apoptosis related cysteine protease antibody, Caspase2 antibody, Caspase-2, Developmentally down regulated 2 antibody, ICH 1 antibody, ICH-1, Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.44 mg/ml
Extra Details:
Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer and other diseases. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse. Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis. The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B. Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7. Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue. Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2. These forms show tissue specificity and function either as inducers or suppressors of apoptosis. Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. Antiserum was purified against the immunizing peptide. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94% homology. Th
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates (lane 2) by Western blot. Asterisk indicates a 48 kDa caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.