Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

GFP Antibody Peroxidase Conjugate

Product Sizes
1 mg
600-303-215-1MG
About this Product
SKU:
600-303-215
Additional Names:
mouse anti-GFP antibody HRP conjugation, peroxidase conjugated mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
9F9.F9
Clonality:
Monoclonal
Conjugate:
HRP
Concentration:
1 mg/ml
Extra Details:
Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Isotype:
IgG, IgG1, kappa
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
GFP Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Western Blot of Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate (p/n 600-303-215) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.
Western Blot of Anti-GFP Monoclonal Antibody Peroxidase Conjugate. Lane 1: Super Signal Molecular Weight Marker. Lane 2: 50ng of GFP. Primary antibody: Mouse Anti-GFP Peroxidase Conjugated Antibody (p/n 600-303-215) at 1:1000 overnight at 2-8°C. Secondary antibody: none. Block: BlockOut - Universal Blocking Buffer (p/n MB-073). Predicted/Observed size: 28kDa for GFP. Other band(s): none.