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Antibodies

GST Antibody

Product Sizes
1 mg
600-101-200-1MG
About this Product
SKU:
600-101-200
Additional Names:
goat anti-GST antibody, Glutathione-S-Transferase
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.06 mg/ml
Extra Details:
Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Glutathione-S-Transferase [Schistosoma japonicum]
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Blue Ice
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Tagged Antibodies
ELISA results of purified Goat Anti-GST Antibody tested against GST. Each well was coated in 1.0 ug of antigen. The starting dilution of antibody was 5 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer (p/n MB-060-1000), Substrate (p/n TMB-8000), and Donkey Anti-Goat IgG Antibody HRP (p/n 605-703-125) at 1:15,000.
Immunoprecipitation of anti-GST antibody. Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 ug per lane. Primary antibody: anti-GST 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Western Blot of Goat Anti-GST Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: rec GST [0.1ug]/ HeLa Whole Cell Lysate [10ug]. Lane 3: rec GST [0.05ug]/ HeLa Whole Cell Lysate [10ug]. Lane 4: rec GST [0.02ug]/ HeLa Whole Cell Lysate [10ug]. Lane 3: HeLa Whole Cell Lysate (p/n W09-000-364) [10ug]. Primary Antibody: Goat anti-GST antibody (p/n 600-101-200) at 1.0ug/mL for overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Goat IgG (H&L) Antibody Peroxidase Conjugated (p/n 605-4302) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) at RT for 30 min. Predicted/Observed size: ~26kDa.
Western Blot of Goat anti-GST antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: CHO/K1 Lysate (p/n W07-000-357). Lane 4: MDA-MB-231 (p/n W09-001-GK6). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: Jurkat Lysate (p/n W09-001-370). Lane 7: NIH/3T3 Lysate (p/n W10-000-358). Lane 8: E-coli HCP Control (p/n 000-001-J08). Lane 9: FLAG Positive Control Lysate (p/n W00-001-383). Lane 10: Red Fluorescent Protein (p/n 000-001-379). Lane 11: Green Fluorescent Protein (p/n 000-001-215). Lane 12: Glutathione-S-Transferase Protein (p/n 000-001-200). Lane 13: Maltose Binding Protein (p/n 000-001-385). Load: 10 ug of lysate or 50ng of purified protein per lane. Primary antibody: GST antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody (p/n 605-4302) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 26 kDa for GST.