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Antibodies

CENP-Q Antibody

Product Sizes
100 ug
200-401-B48-100UG
About this Product
SKU:
200-401-B48
Additional Names:
CENPQ|rabbit anti-CENP-Q Antibody, CenpQ, centromere protein Q, CENP Q
Application:
Detection/Quantitation, ELISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.15 mg/ml
Extra Details:
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex is important for proper recruitment of polo-like kinase 1 (Plk1) to the mitotic kinetochores. A failure in this process results in improper microtubule attachment to the kinetochores and chromosome missegregation that ultimately lead to aneuploidy.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human CENP-Q protein. A BLAST analysis of the full length sequence was used to suggest partial cross-reactivity with CENP-Q based on the following percentage homologies: macaque (91%), horse (76%), bovine (74%), dog (73%), swine (7
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence microscopy using Rockland's protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD
Western blot using Rockland's protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5ug/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus wherein the expression of CENP-Q is expected to not to alter. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus wherein the expression of CENP-Q is knocked down significantly to a level where it is not being detected at all under the tested condition/WB exposure time. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.