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Antibodies

DAD1 Antibody

Product Sizes
100 ug
200-401-AS2-100UG
About this Product
SKU:
200-401-AS2
Additional Names:
DAD1|DAD1 Antibody, OST2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Defender against cell death 1, Oligosaccharyl transferase subunit DAD1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-DAD1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-DAD1 Antibody is affinity chromatography purified via peptide column. Cross reactivity with DAD1 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of DAD1 antibody in HepG2 cell lysate. Lane A: DAD1 antibody at 0.5 ug/mL. Lane B: DAD1 antibody at 1 ug/mL. Lane B: DAD1 antibody at 2 ug/mL. Load: 35 ug per lane. Primary antibody: DAD1 antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 12 kDa, 25 kDa for DAD1. Other band(s): DAD1 splice variants and isoforms.
Immunocytochemistry of DAD1 antibody. Cell Type: HepG2 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: DAD1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DAD1 is located in the endoplasmic reticulum and the cell membrane. Staining: DAD1 is stained brown with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of DAD1 antibody. Cell Type: HepG2 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: DAD1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DAD1 is located in the endoplasmic reticulum and the cell membrane. Staining: DAD1 as red fluorescent signal.