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Antibodies

Streptavidin Antibody

Product Sizes
25 ul
200-401-095S-25UL
About this Product
SKU:
200-401-095S
Additional Names:
rabbit anti-Streptavidin Antibody, rabbit anti Streptavidin
Application:
Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Lateral Flow Assay, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Streptavidin antibody is a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7). With a dissociation constant (Kd) on the order of ~10-14 mol/L,[1] the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively in molecular biology and bio-nanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH. Anti-STREPTAVIDIN antibody is ideal for investigators involved in GFP and Epitope research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Streptavidin [Streptomyces avidinii]
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
Anti-Streptavidin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Stre
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland Rabbit anti Streptavidin (200-4195 lot 23495) and Biotin conjugated Rabbit anti-trypsin inhibitor antibody (200-4679 lot 6594) were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (611-743-127 lot 20831 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc System.
Dot Blot showing the detection of Streptavidin. A three-fold serial dilution of DyLight™ 488 Conjugated Streptavidin starting at 200ng was spotted onto 0.45 um nitrocellulose and blocked in 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Anti-Streptavidin (RABBIT) Antibody (p/n 200-401-095) was incubated in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:1,000 for 1 Hour at 20°C. An HRP Gt-a-Rb secondary antibody (p/n 611-103-122) was incubated at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP.
Western Blot results of Rabbit Anti-Streptavidin Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: Streptavidin (p/n S000-01). Load: 50ng. Primary Antibody: Rabbit Anti-Streptavidin at 1:1000 overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30min at RT. Blocking: BlockOut (p/n MB-073) for 30 min at RT.
Western Blot of Rabbit Anti-Streptavidin Antibody. Lane 1: Streptavidin - Reduced (p/n S000-01) [0.05ug]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Streptavidin - Non-Reduced (p/n S000-01) [0.05ug]. Primary Antibody: Rabbit Anti-Streptavidin at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase (p/n 611-103-122) at 1:70,000 for 30 mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 60 mins at RT. Expect: ~13kDa reduced, ~60kDa non-reduced.