Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Caspase-1 Antibody

Product Sizes
100 ug
200-301-H62-100UG
About this Product
SKU:
200-301-H62
Additional Names:
CASP1|IL1BC, IL1BCE, Caspase-1, CASP-1, 3.4.22.36, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45, Caspase-1 subunit p20, Caspase-1 subunit p10
Application:
Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
14F468
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Anti-Caspase-1 detects human Caspase. Caspase proteins are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspase proteins is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1 (ICE, IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor. Anti-Caspase-1 antibody is ideal for investigators involved in apoptosis research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Caspase-1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids near the c-terminus of the immunogen, human Caspase-1.
Isotype:
IgG1, IgG1, kappa
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-Caspase-1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-1 from human and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Caspase-1 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry of mouse Anti-Caspase-1 antibody. Tissue: human colon cancer. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Caspase-1 at 5 ug/ml for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-1 is a cytoplasmic protein. Staining: Caspase-1 has a DAB chromogen and Hematoxylin counterstain. A). Only rare staining is observed. B). Abundant staining is observed. A and B). Two of these sections are shown at higher magnifications. C). Differential staining was observed.
Western Blot of Mouse Anti-Caspase-1 antibody. Lane A: Human HeLa. Lane B: Mouse NIH3T3 lysate. Load: 30 ug per lane. Primary antibody: Caspase-1 antibody at 0.5 ug/ml and 2 ug/ml respectively for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.2/45 kDa for Caspase-1. Other band(s): none.