BIF1 Antibody
Product Sizes
100 ug
200-301-H61-100UG
About this Product
- SKU:
- 200-301-H61
- Additional Names:
- KIAA0491, Endophilin-B1, Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1|SH3GLB1
- Application:
- Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- 30A882.1.1
- Clonality:
- Monoclonal
- Concentration:
- 1 mg/ml
- Extra Details:
- Anti-Bif-1 Antibody detects human BIF. The Bcl-2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl-2, Bcl-XL and pro-apoptotic (or cell death) genes including Bax and BAD. In addition to its anti-apoptotic effect, Bcl-2 also inhibits progression through the cell cycle. Functional interactions between family members as well as binding to other cellular proteins modulate their activities. Bcl-2 family members are important in normal tissue development, homeostasis, and disease states. Bcl-2 prevents cell death by forming heterodimers with Bax and Bad. Bax promotes cell death by forming homodimers, which can be blocked by forming heterodimers with Bcl-2 or BCL-XL. Recently, using yeast two-hybrid system Cuddeback have identified a cDNA encoding a 365 amino acid protein, which interacts physically with Bax protein and influences cell life and death. Bif-1 contains a Src homology 3 (SH3) domain in the C-terminus. It does not contain any of the conserved Bcl-2 homology (BH) domains of the Bcl-2 family proteins. Human Bif-1 protein shares 96% and 42% identity at the amino acids level with mouse and C. elegans, respectively. Overexpression of Bif-1 in transfected cell lines promotes Bax conformational change, caspase activation, and apoptotic cell death following growth factor withdrawal. Bif-1 RNA is abundantly expressed in heart, skeletal muscle, kidney, and placenta. Anti-BIF-1 antibody is ideal for investigators involved in cytokines and growth factor research and NFkappaB and signaling research.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Mouse
- Immunogen:
- Bif-1 Antibody was produced in mice and was prepared by repeated immunizations at the c-terminus of the human recombinant protein Bif-1.
- Isotype:
- IgG1, IgG1, kappa
- Physical State:
- Liquid
- Reactivities:
- Human
- Shipping Conditions:
- Dry Ice
- Specificity:
- Anti-Bif-1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Bif-1 from Human based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Bif-1 from other sources has not been determined.
- Storage Conditions:
- -20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Monoclonal Antibody
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=200-301-H61
