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Antibodies

Carboxypeptidase A Antibody

Product Sizes
2 ml
100-4152-2ML
About this Product
SKU:
100-4152
Additional Names:
CPA1|rabbit anti-Carboxypeptidase A Antibody, Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody
Application:
Detection/Quantitation, ELISA, FISH, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
90 mg/ml
Extra Details:
Anti-Carboxypeptidase A antibody is specific for Carboxypeptidase A enzyme. Carboxypeptidase A is a pancreatic exopeptidase which hydrolyzes peptide bonds of C-terminal residues with aromatic or aliphatic side chains. This enzyme is also commonly called CPA1, where a related pancreatic carboxypeptidase is CPA2 . There are several related enzymes referred to as CPA 3 through CPA6.
Format:
Antiserum
Formulation:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Carboxypeptidase A [Bovine Pancreas]
Physical State:
Lyophilized
Reactivities:
Bovine
Shipping Conditions:
Ambient
Specificity:
Carboxypeptidase A antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Carboxypeptidase A [Bovine Pancreas]. Cross reactivity against Carboxypeptidase A from other tissues and species may occur but have not been specifically determined.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
ISH results using Anti-Carboxypeptidase A Antibody. (A) Fluorescence images showing the endocrine (RFP) and exocrine pancreas (GFP). When each transgenic fish is crossed with Ins-DsRed zebrafish, the development of insulin-expressing endocrine pancreas is not impaired by Hh over-expression. A smaller dot-like insulin-positive structure (white arrowheads) which corresponds to the anterior endocrine cells is observed in approximately half of the control and Hh-expressing embryos. (B, C) Whole mount ISH for trypsin, elastase, and carboxypeptidase A (CPA) at different time points. Over-expression of Hh ligands does not compromise the exocrine differentiation of the zebrafish pancreas, as evidenced by the proper and timely expression of trypsin. Expression of the other exocrine markers is also unaffected by Hh over-expression. Hh over-expression, however, induces subtle morphologic changes of the exocrine pancreas, showing a short, slender, and tortuous posterior pancreas compared to those of controls, which is evident by ISH for exocrine markers at 4 and 5 dpf and exaggerated at 12 dpf. (D) Confocal images of immunofluorescence staining for CPA. Regardless of transgene (GFP) expression, most acinar cells express CPA, suggesting unaffected exocrine differentiation by Hh over-expression. Lat., lateral. Fig. 2. PMID: 22164219.