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Antibodies

CD31 Antibody

Product Sizes
25 ul
100-401-GN4S-25UL
About this Product
SKU:
100-401-GN4S
Additional Names:
Pecam1|rabbit anti-CD31 Antibody, rabbit anti-PECAM-1 antibody, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31 Antibody, PECAM1 Antibody, CD31/PECAM1 Antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
90 mg/ml
Extra Details:
Anti-CD31 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). CD31 (PECAM-1) is a 130 kDa platelet endothelial cell adhesion molecule encoded by the PECAM1 gene found on chromosome 17 in humans. CD31 is expressed on platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 plays a key role in modulation of integrin-mediated cell adhesion, leukocyte transendothelial migration, angiogenesis, apoptosis and macrophage phagocytosis. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. Anti-CD31 is ideal for researchers interested in Stem Cell Research, Epigenetics, and Cell-cycle Regulation research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
CD31 PECAM1 antibody is directed against rat CD31 protein. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with CD31 from rat, human, and mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit Anti-CD31 Antibody. Lane 1: 3T3 Whole cell lysate (p/n W10-000-358). Lane 2: Jurkat Whole cell lysate (p/n W09-001-370). Lane 3: HT-29 Whole cell lysate (p/n W09-001-BS0). Load: 10ug/lane. Primary Antibody Anti-CD-31 used 1:500 with 0.75% TBS with Casein overnight. Secondary Antibody Goat anti-rabbit HRP (p/n 611-103-122) used 1:40,000 for 30 min at room temp. Expect MW: 80kda.
Immunohistochemistry with anti-CD31 antibody showing CD31 staining of vascular endothelium in human colon at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-human CD31 antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3'3-diamino-benzidine substrate (DAB).