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Antibodies

CDC2 p34 Antibody

Product Sizes
100 ul
100-401-160-100UL
About this Product
SKU:
100-401-160
Additional Names:
CDK1|rabbit anti-Cdc2 antibody, rabbit anti-p34 antibody, Cell Division Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDK1, CDC2, CDC28A, CDKN1, P34CDC2
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
None
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
85 mg/ml
Extra Details:
p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF). The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues. In humans the phosphorylation appears to be performed by p60src. The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins. The phosphorylation activity is coupled to the entry into the M-phase of the cell. p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally. Association with deletion mutants of cyclin halts the M-phase before it is completed.
Format:
Antiserum
Host:
Rabbit
Immunogen:
CDC2 peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 34 kDa cdc2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdc2 from other species may also occur.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland's anti-cdc2 Cyclin Dependent Kinase was used to detect human p34cdc2 by western blot in untreated (Control) and drug treated lysates of MCF-7 cells. Lane 1-4 represents 3.1uM, 6.2uM, 12.5uM and 25.0uM genistein treatment of cells before lysates were prepared. Detection occurs using a 1:1,000 dilution. Although this antiserum detects non-specific bands at higher MW, a clear induction of signal is observed as the concentration of drug is increased. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.